Composite
Part:BBa_K3362001:Design
Designed by: Marvin Skiba Group: iGEM20_Hamburg (2020-10-25)
Target cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 903 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 29
Illegal NheI site found at 52
Illegal SpeI site found at 904
Illegal PstI site found at 918
Illegal NotI site found at 7
Illegal NotI site found at 911 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 904 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 904
Illegal PstI site found at 918 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No we did not any considerations.
Source
The target cassette was artificially created.